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PKC iota antibody (AA 215-264)

This Rabbit Polyclonal antibody specifically detects PKC iota in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey and Pig.
Catalog No. ABIN6745618

Quick Overview for PKC iota antibody (AA 215-264) (ABIN6745618)

Target

See all PKC iota (PRKCI) Antibodies
PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity

Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig

Host

  • 29
  • 4
Rabbit

Clonality

  • 29
  • 4
Polyclonal

Conjugate

  • 14
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PKC iota antibody is un-conjugated

Application

  • 32
  • 15
  • 13
  • 13
  • 10
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 215-264

    Specificity

    Human PRKCI / PKC-Iota

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Horse, Pig, Guinea pig (100%) Chicken (92%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa215-264 of human PRKCI (P41743, NP_002731). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Lizard (92%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target

    PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

    Alternative Name

    PRKCI / PKC Iota

    Background

    Name/Gene ID: PRKCI
    Subfamily: PKC
    Family: Protein Kinase

    Synonyms: PRKCI, APKC-lambda/iota, DXS1179E, PKC iota, Protein kinase C, iota, Protein kinase C iota type, NPKC-iota, PKCI, PRKC-lambda/iota, Protein kinase C iota

    Gene ID

    5584

    NCBI Accession

    NP_002731

    UniProt

    P41743

    Pathways

    Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation
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